Engineering subtilisin proteases that specifically degrade active RAS
نویسندگان
چکیده
منابع مشابه
Focal cerebral ischemia induces active proteases that degrade microvascular matrix.
BACKGROUND AND PURPOSE Focal cerebral ischemia causes microvessel matrix degradation and generates proteases known to degrade this matrix. However, proof that the proteases generated do indeed degrade vascular matrix is lacking. Here we demonstrate that active proteases derived from ischemic tissue after middle cerebral artery occlusion (MCAO) and transferred to normal tissue can degrade vascul...
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Parasitic protozoa are among the most important pathogens worldwide. Diseases such as malaria, leishmaniasis, amoebiasis, giardiasis, trichomoniasis, and trypanosomiasis affect millions of people. Humans are constantly threatened by infections caused by these pathogens. Parasites engage a plethora of surface and secreted molecules to attach to and enter mammalian cells. The secretion of lytic e...
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Fibrinolytic enzyme produced by Bacillus spp. are known to possess an unique property to degrade fibrin blood clots. Fibrinolytic enzyme such as nattokinase has commercial applications as a therapeutic agents and functional food formulation. In this study, we report an interesting characteristic feature of Bacillus subtilis BR21, a native isolate for its ability to produce multiple proteases th...
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Baker's yeast was found to contain inhibitors of yeast proteases A and C. These two proteins were partially purified, characterized, and compared with the previously described inhibitor of protease B. The A and B inhibitors were very thermostable and were extracted from intact yeast cells at 9k C. The A inhibitor appeared to be a protein with a molecular weight of about 22,000 which could be di...
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ژورنال
عنوان ژورنال: Communications Biology
سال: 2021
ISSN: 2399-3642
DOI: 10.1038/s42003-021-01818-7